Data Question At what temperature does an Elodea plant photosynthesize at the best? Continue to take readings every 5 minutes for one hour. This extract is probably too concentrated to use directly in the spectrophotometer, so you will have to dilute it. In this lab, you will investigate the process of photosynthesis. Analysis of Photosynthetic Pigments The pigments identified above (chlorophylls a and b, carotenoids, and xanthophylls) can be extracted and separated for analysis by several methods. Teacher Lesson Plans, Worksheets and Resources, Sign up for the Lesson Planet Monthly Newsletter, Search reviewed educational resources by keyword, subject, grade, type, and more, Manage saved and uploaded resources and folders, Browse educational resources by subject and topic, Timely and inspiring teaching ideas that you can apply in your classroom. 7. Elodea plant, the chemical . Summary: This lab that we did was to see what would happen to see what would happen to the Elodea plant if we put a different substance in our beakers. Photosynthesis in Elodea Lab Pictures Hypothesis If we place an Elodea plant in hot water then it will produce photosynthesize better because it will produce more bubbles Conclusion Materials F4 G4 d4 s4 Â4 Ã4 j5 ı û û ı û ı ı ı ı û û û û û û û ı û û ı û H*>* + " s � � Ÿ ¾ ¿ C Videos (Over 2 Million Educational Videos Available). Other. Your lab group is asked to design, execute, and analyze an experiment that tries to accomplish the following two tasks: Task A. Each group of students should obtain two manometer tubes. Save. ĞÏࡱá > şÿ ? . Continue this process, in 25 nm increments, until you reach 700 nm. To this add a very small amount (1 scoop) of CaCO3 and a small (3 scoops amount of fine quartz sand (use the scoops provided for the CaCO3 and sand). . by benjaminm. Elodea. In this photosynthesis lesson students answer lab questions, complete a web quest, take a quiz and a test. Showing top 8 worksheets in the category - Photosynthesis And Respiration In Elodea. Photosynthesis is a complex anabolic process that occurs in the chloroplasts of plant leaves. Remove several leaves from around the cut end of the stem. For this photosynthesis lesson plan, students place elodea in a test tube filled with water. One group in class should use red light as the energy source, another group should use green light, and a third group should use white light. A post-lab class discussion would include the sharing of information between the two groups. The independent variable is? Data Analysis: 1. Put the top on the chamber and set it aside to allow the vapor phase to equilibrate. Summarize the process of photosynthesis. Egeria densa, also known as Elodea densa because of its previously incorrect classification in the Elodea genus, is the most popular aquatic plant used to teach photosynthesis in the classroom. A comprehensive online edtech PD solution for schools and districts. This exchange of gases between the atmosphere and the leaves of terrestrial plants occurs through microscopic openings on the leaf surface called stomata. Plants perform photosynthesis to create sugars and other organic compounds that they need to survive. Gently wave the strip about 4 or 5 times in the air to accelerate drying of the spot. Allow the chromatogram to “run” (about 10 minutes) until the solvent moves to within 2 cm of the top and then remove the strip, being sure to replace the top. Use the syringe to force some bicarbonate solution into the pipettes and note . 1. Plants themselves depend on photosynthesis as a novel method of producing carbohydrate. Obtain a hematocrit capillary tube and, holding the red-marked end in your hand, draw some of the pigment extract, which you have just prepared, into the tube. In its simplest form, photosynthesis captures the energy of sunlight and uses this to drive the formation of carbohydrates. 0. This lab can be done as a demonstration to begin the unit, or it can done as an inquiry lab where students try to devise an explanation for the … Can you determine from your data which pigments are responsible for this absorption spectrum? Photosynthesis in plants generally involves the green pigment chlorophyll and generates oxygen as a by-product. Share this science project . A beaker of water serves as an efficient heat sink. 6. Earth science pupils test animal, plant, and fossil fuels as sources in this investigation. Allow the spot to dry before applying a repeat spot. We will do a simple chemical extraction using an acetone-water solvent followed by filtration to remove tissue debris. students will be able to "see" photosynthesis in action as colors change (or don't) using Elodea, bromothymol blue, and carbon dioxide.This is a 3 page lab with background info, materials list, clear pr 7. In the second part, you will determine with a spectrophotometer what wavelengths of visible light are actually absorbed by plant pigments. Remove the sample tube, set the wavelength to 425 nm and proceed, as before, to measure and record absorbance. Place both tubes in a test-tube rack. 2 years ago. IB Biology design lab using Elodea canadensis. Life science teams experiment by placing sprigs of Elodea into a sodium bicarbonate solution and counting the oxygen bubbles produced. D ®. 4. Œ' œ' p* q* ˆ* ‰* –* —* + + ., /, h, i, - - 1- G- ­. Bromothymol Blue (which acts as an indicator to show if photosynthesis is occurring). Bromthymol blue is used as an indicator for the presence of CO2 in solution. No matter your students' level, we have an activity to stretch their scientific knowledge. B. Chromatography of extracted pigments. Pour the slurry into the Buchner and gently draw the liquid extract into the flask by turning on the cold water faucet Rinse with 25 ml of acetone water solution. Educators earn digital badges that certify knowledge, skill, and experience. The spot should be as dense as possible, but no larger than 3-4 mm. Demonstrate that environmental CO2 is used during photosynthesis in Elodea. . Elodea Lab DRAFT. Mar 23, 2019 - Explore Patricia Segill's board "Photosynthesis lab" on Pinterest. Fortunately, you don’t have to prepare the thin-layer medium; plastic strips on which silica gel has been pre-spread are available for this exercise. Your little scientists will discover how plants grow with these photosynthesis worksheets and printables! After 5 minutes, record the position of the fluid in the manometer. Using a technique called chromatography you will then separate the various pigments based on differences in their molecular structures. Use this tube as a control to ensure that your measurements of photosynthesis are reliable. It is the absorbed light energy that can do useful work in photosynthesis. In aquatic ecosystems, primary producers include photosynthetic bacteria, phytoplankton (single-celled photosynthetic aquatic organisms), multicellular algae, and vascular plants, such as Elodea sp. The rates of migration for individual compounds differ according to the chemical structure of the compounds, the nature of the chromatographic medium, and physical factors such as pH and temperature. They consider plant production of carbon dioxide and the use of live materials in biological... Students investigate how plants absorb carbon dioxide during photosynthesis. IntroductionThis lab has been created in order to find what extent does distance from a light source (5cm, 10cm, and 15cm) affect the rate of photosynthesis (measured in bubbles / 3 min) in Elodea … The colors of the pigments that you should have extracted from spinach leaves earlier could be generally described as follows: chlorophyll a- blue-green chlorophyll b - yellow-green carotenoids- yellow xanthophylls- greenish-yellow Re-examine your thin-layer chromatogram. Intermediate High School. Simple experiment to demonstrate oxygen production in pond weed. Demonstrate that there is a net production of CO2 when Elodea respires in the absence of photosynthesis. Data Analysis: 1. These stoppers will be fit with a bent section of a graduated pipette. We will now examine one technique that can be used to characterize pigments by the specific wavelengths of light that they absorb. Wrap one tube with aluminum foil. Elodea plant, the chemical . Your maximum absorbance values should be in the range of 0.4 to 1 .4. Young scientists use eldoea plants to observe the oxygen production from photosynthesis. In this lab, you will investigate the process of photosynthesis. 1. IntroductionThis lab has been created in order to find what extent does distance from a light source (5cm, 10cm, and 15cm) affect the rate of photosynthesis (measured in bubbles / 3 min) in Elodea water plants. ‘! Data is recorded and graphed on a Google spreadsheet, making this not just a lesson in photosynthesis,... Photosynthesis is the literal light snack. 6. Task B. Animals at all other trophic levels also depend, either directly or indirectly, on the organic molecules that comprise plant tissue. Here is a lesson that includes a short introductory presentation, two virtual lab experiments, a summary presentation, and accompanying worksheet for all. Describe, in general, a procedure that would allow you to identify the absorption spectrum of each of the pigments in your extract. Pour the liquid into a small, dark screw top glass bottle and save for chromatographic and spectrophotometric analysis. More specifically, you will learn how the amount of light affects photosynthesis. Elodea Design Lab - Free download as Word Doc (.doc / .docx), PDF File (.pdf), Text File (.txt) or read online for free. Lightly pack each tube with equal amounts of fresh Elodea; try to pick single, healthy stems and insert them with the shoot tip toward the bottom of the tube. They study the difference between having access to high amounts of light and low amounts... Middle schoolers use elodea to determine the rate of photosynthesis. Reset the manometer to zero using the syringes. Each group of students should obtain about 15 g of spinach leaves (about 4 leaves). 91 Exercise 1 – Observing Photosynthesis via CO 2 Consumption 1. 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